##FastQC 0.11.2
>>Basic Statistics pass
#Measure Value
Filename C_cutadapt_2nd.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24600690
Sequences flagged as poor quality 0
Sequence length 6-88
%GC 64
>>END_MODULE
>>Per base sequence quality pass
#Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile
1 37.910978757099905 39.0 38.0 39.0 35.0 39.0
2 37.923354832730304 39.0 38.0 39.0 35.0 39.0
3 37.9283379449926 39.0 38.0 39.0 35.0 39.0
4 37.88846723404912 39.0 38.0 39.0 35.0 39.0
5 39.3104496662492 41.0 39.0 41.0 36.0 41.0
6 39.284012074458076 41.0 39.0 41.0 36.0 41.0
7 39.25957338162054 41.0 39.0 41.0 35.0 41.0
8 39.22624735086212 41.0 39.0 41.0 35.0 41.0
9 39.1715033276574 41.0 39.0 41.0 35.0 41.0
10-11 39.148177716958074 41.0 39.0 41.0 35.0 41.0
12-13 38.992706956485804 40.5 38.0 41.0 35.0 41.0
14-15 38.7349278783089 40.0 38.0 41.0 35.0 41.0
16-17 38.58290918354951 40.0 37.5 41.0 35.0 41.0
18-19 38.32620635012595 40.0 37.0 41.0 34.0 41.0
20-21 38.0134099481602 40.0 35.5 41.0 33.5 41.0
22-23 37.72317215391014 39.5 35.0 41.0 33.0 41.0
24-25 37.385117980117386 39.0 35.0 41.0 33.0 41.0
26-27 37.101601216133616 38.0 35.0 41.0 33.0 41.0
28-29 36.849816755743326 37.5 35.0 41.0 33.0 41.0
30-31 36.58747709434698 37.0 35.0 41.0 33.0 41.0
32-33 36.24908005010832 35.5 35.0 40.0 31.5 41.0
34-35 36.034137251944685 35.0 35.0 40.0 31.0 41.0
36-37 35.88874299680135 35.0 35.0 40.0 31.0 41.0
38-39 35.66284963282469 35.0 35.0 40.0 31.0 41.0
40-41 35.4455564773664 35.0 34.5 40.0 31.0 41.0
42-43 35.30519283838838 35.0 34.0 39.0 31.0 41.0
44-45 35.0752768984797 35.0 34.0 39.0 30.5 41.0
46-47 34.865980260971185 35.0 34.0 39.0 30.0 41.0
48-49 34.623708728003834 35.0 33.0 38.0 29.0 41.0
50-51 34.50598004937264 35.0 33.0 37.0 28.5 41.0
52-53 34.23801298519996 35.0 33.0 37.0 27.0 40.0
54-55 33.96155546762118 35.0 33.0 36.0 27.0 40.0
56-57 33.85291370375249 35.0 33.0 35.5 28.5 40.0
58-59 33.668232207491116 35.0 33.0 35.0 27.5 39.0
60-61 33.48931918284882 35.0 33.0 35.0 27.0 39.0
62-63 33.1386378479818 35.0 33.0 35.0 26.0 38.5
64-65 32.84963007556274 35.0 33.0 35.0 26.0 37.0
66-67 29.10149195496986 31.0 26.5 33.0 19.5 35.0
68-69 30.4792051119248 32.0 28.5 34.5 22.0 35.5
70-71 32.083591932313105 34.5 31.5 35.0 24.0 36.0
72-73 32.24479428115002 35.0 32.0 35.0 24.5 35.5
74-75 32.01291147836827 35.0 32.0 35.0 24.0 35.0
76-77 31.715989667983905 35.0 31.5 35.0 24.0 35.0
78-79 31.27383454401351 34.5 31.0 35.0 23.0 35.0
80-81 31.154415641670788 34.0 31.0 35.0 23.5 35.0
82-83 30.9187528686603 34.0 31.0 35.0 21.5 35.0
84-85 30.705309984163037 34.0 31.0 35.0 20.0 35.0
86-87 30.145640326161914 34.0 31.0 35.0 18.5 35.0
88 29.548656205747474 34.0 30.0 35.0 7.0 35.0
>>END_MODULE
>>Per tile sequence quality pass
#Tile Base Mean
2306 1 0.24099566969941577
2306 2 0.2776247403553924
2306 3 0.2919505495680852
2306 4 0.2977797457228135
2306 5 0.3914388956805013
2306 6 0.37571533657789047
2306 7 0.3576289353182247
2306 8 0.3136817390542461
2306 9 0.36471992127266617
2306 10-11 0.3152974143370102
2306 12-13 0.3087918362830564
2306 14-15 0.41438419478024713
2306 16-17 0.4341267368728978
2306 18-19 0.42752618759589467
2306 20-21 0.40977072342976584
2306 22-23 0.4644678153149471
2306 24-25 0.4469572302825142
2306 26-27 0.4708690544434333
2306 28-29 0.46849161526046856
2306 30-31 0.4618397371631744
2306 32-33 0.45855777085230187
2306 34-35 0.4269488786266322
2306 36-37 0.44084562296653473
2306 38-39 0.34089338087994037
2306 40-41 0.3880922165329892
2306 42-43 0.2870171056057558
2306 44-45 0.22518893256903283
2306 46-47 0.23920518820205672
2306 48-49 0.29135221591833727
2306 50-51 0.26378689706947966
2306 52-53 0.1985181545010235
2306 54-55 0.06869399705723112
2306 56-57 -0.08856733693495045
2306 58-59 -0.08332217079536264
2306 60-61 -0.08602863666755667
2306 62-63 -0.23192835454243976
2306 64-65 -0.29971362883385666
2306 66-67 -1.3224673592837668
2306 68-69 -1.195687671434321
2306 70-71 -0.573127551544161
2306 72-73 -0.44529323458077386
2306 74-75 -0.4726372463061921
2306 76-77 -0.7289242877969571
2306 78-79 -0.8440029694611084
2306 80-81 -1.078880335654059
2306 82-83 -1.0810402119467248
2306 84-85 -1.3504275394211973
2306 86-87 -1.4836022392091195
2306 88 -1.7166816529873223
2307 1 0.24986495872883552
2307 2 0.2509578142374167
2307 3 0.2433548401568757
2307 4 0.2629281813018167
2307 5 0.40831880242134844
2307 6 0.3172839700006236
2307 7 0.33583460442628876
2307 8 0.26415110384813545
2307 9 0.34805701307754333
2307 10-11 0.31837513190909306
2307 12-13 0.3758712709791183
2307 14-15 0.43094416998041396
2307 16-17 0.3723335223781348
2307 18-19 0.4236260195832031
2307 20-21 0.39003310612697817
2307 22-23 0.4130368389956516
2307 24-25 0.4212348606139287
2307 26-27 0.43815364248099087
2307 28-29 0.473349524939799
2307 30-31 0.4894041732937353
2307 32-33 0.5469472654951488
2307 34-35 0.4681311247788642
2307 36-37 0.36369212980853405
2307 38-39 0.3450520429233137
2307 40-41 0.359322616392852
2307 42-43 0.2536220946788603
2307 44-45 0.28034548983932694
2307 46-47 0.2512193544482173
2307 48-49 0.2585564997390506
2307 50-51 0.24127037076763003
2307 52-53 0.17067041326214394
2307 54-55 0.17581007653075886
2307 56-57 0.13230476379499123
2307 58-59 0.027923804743210212
2307 60-61 -0.0681730839295085
2307 62-63 -0.17070544027226475
2307 64-65 -0.14248626737823145
2307 66-67 -1.1079571443755611
2307 68-69 -0.9531841884315746
2307 70-71 -0.46361247770927605
2307 72-73 -0.39860534155095095
2307 74-75 -0.4363047287319617
2307 76-77 -0.5299242965364712
2307 78-79 -0.6991101563861193
2307 80-81 -0.7825293580272756
2307 82-83 -0.9845133716695713
2307 84-85 -1.1445125026781504
2307 86-87 -1.1861332335924857
2307 88 -1.354369829185078
2201 1 0.2587939434146449
2201 2 0.22247862832430343
2201 3 0.24764027475421102
2201 4 0.24386123776153568
2201 5 0.36933278647716605
2201 6 0.3442961992558864
2201 7 0.30419130081165235
2201 8 0.25381791402658394
2201 9 0.29244941437412564
2201 10-11 0.29899932506428684
2201 12-13 0.3179403042422777
2201 14-15 0.355984189407458
2201 16-17 0.35468963379729956
2201 18-19 0.35925023948751544
2201 20-21 0.3344198875280924
2201 22-23 0.3495666950379288
2201 24-25 0.4222071428116294
2201 26-27 0.38230564153331414
2201 28-29 0.3827020128266838
2201 30-31 0.3701949610246871
2201 32-33 0.3896072853721293
2201 34-35 0.35142223200838885
2201 36-37 0.2834164463653508
2201 38-39 0.29593313901023066
2201 40-41 0.22691887625134655
2201 42-43 0.14767842169413115
2201 44-45 0.09086159049932263
2201 46-47 0.11449552497142435
2201 48-49 0.13201576244961188
2201 50-51 0.05830549302900323
2201 52-53 0.061445601851417564
2201 54-55 0.0572663869979948
2201 56-57 -0.01617647515021048
2201 58-59 0.004785875540207485
2201 60-61 -0.042291745907441225
2201 62-63 -0.11709139349198239
2201 64-65 -0.22914701900455725
2201 66-67 -0.7694058226791682
2201 68-69 -0.6121840482272098
2201 70-71 -0.46145506355644983
2201 72-73 -0.38951207056378223
2201 74-75 -0.44548824317514857
2201 76-77 -0.5119778832235156
2201 78-79 -0.6610838253494222
2201 80-81 -0.838270710892612
2201 82-83 -0.8994512799827241
2201 84-85 -0.9203575177146561
2201 86-87 -1.0124714384046953
2201 88 -1.2538120585583918
1101 1 0.17832957568875685
1101 2 0.1539231387515443
1101 3 0.18725373302658
1101 4 0.21698236297855544
1101 5 0.23582433971288452
1101 6 0.2760288177842156
1101 7 0.2868664007665842
1101 8 0.22809635880682322
1101 9 0.23802703940342695
1101 10-11 0.1945085477653663
1101 12-13 0.25291012585095274
1101 14-15 0.2328437045633862
1101 16-17 0.2855916239699283
1101 18-19 0.3075158684137591
1101 20-21 0.27290975940000806
1101 22-23 0.22090248871999307
1101 24-25 0.27265402312655596
1101 26-27 0.25376986038054383
1101 28-29 0.260349585631829
1101 30-31 0.2095325507964887
1101 32-33 0.25979448422086193
1101 34-35 0.291901022398811
1101 36-37 0.20817311452319132
1101 38-39 0.18372983796550812
1101 40-41 0.14503183638371553
1101 42-43 0.14036863534657584
1101 44-45 0.15289970512593243
1101 46-47 0.13401173172501046
1101 48-49 0.060040123100307596
1101 50-51 0.043774694408121206
1101 52-53 0.06634779369829857
1101 54-55 0.01608173050992434
1101 56-57 -0.01608100384610367
1101 58-59 -0.010446964612611964
1101 60-61 -0.030689270014541137
1101 62-63 -0.13248699155980148
1101 64-65 -0.12593550331168757
1101 66-67 -0.8064493588431745
1101 68-69 -0.5251826193375919
1101 70-71 -0.24776936317179477
1101 72-73 -0.2747474900626017
1101 74-75 -0.31486211970693034
1101 76-77 -0.3102908651481755
1101 78-79 -0.3605114346758391
1101 80-81 -0.5103295074129193
1101 82-83 -0.600621874175733
1101 84-85 -0.6826308914469301
1101 86-87 -0.7033960191463073
1101 88 -0.79222601445883
2304 1 0.1895838652420565
2304 2 0.1964925280619454
2304 3 0.1771159091776724
2304 4 0.2307819694326838
2304 5 0.27637110163750833
2304 6 0.19972666037347864
2304 7 0.18626465784591772
2304 8 0.19086561189406126
2304 9 0.23932626266771706
2304 10-11 0.22824003580316798
2304 12-13 0.2717302226226366
2304 14-15 0.21624561002128928
2304 16-17 0.24284808214137854
2304 18-19 0.25290357106263883
2304 20-21 0.23070334677242954
2304 22-23 0.22414299268503868
2304 24-25 0.28995499655325574
2304 26-27 0.25558224903750215
2304 28-29 0.2477781791703464
2304 30-31 0.21711983209151953
2304 32-33 0.2397378305520519
2304 34-35 0.2388413640305771
2304 36-37 0.229018402251981
2304 38-39 0.16330926821826353
2304 40-41 0.09522225699468123
2304 42-43 0.1109387914568245
2304 44-45 0.17765848393745642
2304 46-47 0.1678874218031794
2304 48-49 0.11917312740929731
2304 50-51 0.09639605634986737
2304 52-53 0.10807743670545733
2304 54-55 0.14827828936555676
2304 56-57 0.0927673345856519
2304 58-59 0.07936235677527748
2304 60-61 -0.0210069541192226
2304 62-63 -0.10888284104215273
2304 64-65 -0.07479676054435913
2304 66-67 -0.7342996547149028
2304 68-69 -0.43849048879606656
2304 70-71 -0.08148365714382422
2304 72-73 -0.07304365968283122
2304 74-75 -0.029439048451123995
2304 76-77 -0.07398625663098812
2304 78-79 -0.09916339946379082
2304 80-81 -0.05427037578165894
2304 82-83 -0.08363143431932585
2304 84-85 -0.1328815526469711
2304 86-87 -0.18100271369834076
2304 88 -0.16055210033264444
2202 1 0.150775263395019
2202 2 0.1524776771934384
2202 3 0.11953016362032542
2202 4 0.15373801354907357
2202 5 0.16779816371357725
2202 6 0.1476637472017046
2202 7 0.16287245335350775
2202 8 0.16615459202262173
2202 9 0.16834218890456754
2202 10-11 0.16141618536699553
2202 12-13 0.1544352579335566
2202 14-15 0.18106889486902844
2202 16-17 0.18503557388470426
2202 18-19 0.18139326593080796
2202 20-21 0.17834236460393527
2202 22-23 0.14617028582640756
2202 24-25 0.15246122571537768
2202 26-27 0.1550138077811951
2202 28-29 0.1361938098201918
2202 30-31 0.13435058468999017
2202 32-33 0.16231513381541873
2202 34-35 0.1389734952509727
2202 36-37 0.1230741632841017
2202 38-39 0.11716731370525935
2202 40-41 0.12511389254343186
2202 42-43 0.10966760904710071
2202 44-45 0.12141280082634154
2202 46-47 0.11801386856900109
2202 48-49 0.1259807557750534
2202 50-51 0.0899344006514724
2202 52-53 0.07014738490577344
2202 54-55 0.11222510610596004
2202 56-57 0.06405140225843553
2202 58-59 0.07840137123338309
2202 60-61 0.014240993429460502
2202 62-63 -0.0703016100673608
2202 64-65 -0.03936422571982945
2202 66-67 -0.43753486170810874
2202 68-69 -0.15602612516051906
2202 70-71 -0.09085190006004851
2202 72-73 -0.06644604224851491
2202 74-75 -0.069124388228758
2202 76-77 -0.053898571004154405
2202 78-79 -0.11506664152885904
2202 80-81 -0.21224636320459922
2202 82-83 -0.18177328009008775
2202 84-85 -0.20937666434851465
2202 86-87 -0.17908184104777547
2202 88 -0.2532111410979496
1102 1 0.08052637071334345
1102 2 0.05814572412621999
1102 3 0.047375922571603724
1102 4 0.09679390871431082
1102 5 0.13172733504634238
1102 6 0.11661963714001189
1102 7 0.12063259328679266
1102 8 0.10274001706683578
1102 9 0.08820987194364704
1102 10-11 0.09461676439804023
1102 12-13 0.04101597642479504
1102 14-15 0.08037530985270536
1102 16-17 0.06739496567267622
1102 18-19 0.07905408786041335
1102 20-21 0.04311302846249987
1102 22-23 0.04073612456173947
1102 24-25 0.08296527450209368
1102 26-27 0.08605209803923941
1102 28-29 0.03931019041739603
1102 30-31 0.10250563286724201
1102 32-33 0.056743202252789615
1102 34-35 0.08701949648617102
1102 36-37 0.0633347399069848
1102 38-39 0.06284691017415156
1102 40-41 0.07526752352604404
1102 42-43 0.08231664039936248
1102 44-45 0.09856373410968189
1102 46-47 0.01491311577859733
1102 48-49 0.015410131093432256
1102 50-51 0.08289640946309618
1102 52-53 0.06026870997810363
1102 54-55 0.05220668527682193
1102 56-57 0.004629611698355518
1102 58-59 0.03635096382971881
1102 60-61 0.07177237753973031
1102 62-63 -0.07554440013296215
1102 64-65 -0.08697903136368978
1102 66-67 -0.4097525497667398
1102 68-69 -0.2572791309379916
1102 70-71 -0.06674965228694063
1102 72-73 0.02698945418620724
1102 74-75 -0.054669144932255165
1102 76-77 0.006992899914134654
1102 78-79 -0.10677919724587781
1102 80-81 -0.12748018430754016
1102 82-83 -0.09378819427199758
1102 84-85 -0.13246210574660822
1102 86-87 -0.14693924850405793
1102 88 -0.21664178780848076
2305 1 0.02119181992321728
2305 2 0.03714348173693338
2305 3 0.026847758403043542
2305 4 0.02160208471863001
2305 5 0.04733945526647432
2305 6 0.041879011544807554
2305 7 0.06707827937187005
2305 8 0.05809366567735452
2305 9 0.04653949290110404
2305 10-11 0.02613480002263202
2305 12-13 0.04190949439071545
2305 14-15 0.06385658313127607
2305 16-17 0.03788488903836651
2305 18-19 0.03773032384610531
2305 20-21 0.02370486041431974
2305 22-23 0.02921371668008277
2305 24-25 0.03840761946292304
2305 26-27 0.00835903797462123
2305 28-29 0.014757532107381621
2305 30-31 0.016861283679055816
2305 32-33 0.036978668912503565
2305 34-35 -0.007750591803755924
2305 36-37 -0.01289570126855466
2305 38-39 -0.0019123196345987026
2305 40-41 0.038455270112542905
2305 42-43 -0.011176825810359503
2305 44-45 0.009113993385049923
2305 46-47 0.04327060020714413
2305 48-49 0.09289130923297506
2305 50-51 0.10453684044255596
2305 52-53 0.05754380399075387
2305 54-55 -0.025633322797212088
2305 56-57 -0.02564670329877572
2305 58-59 0.05052488213682693
2305 60-61 0.06399465065607757
2305 62-63 -0.04706034995030706
2305 64-65 -0.016050246712133287
2305 66-67 -0.5094027605340941
2305 68-69 -0.2801748393810506
2305 70-71 -0.021634901705446907
2305 72-73 0.05233323431707504
2305 74-75 0.1123691437422849
2305 76-77 0.1336938117639903
2305 78-79 0.03981620653795659
2305 80-81 0.1103911693410673
2305 82-83 0.09001063123541186
2305 84-85 0.12844150675023158
2305 86-87 0.07909886518118725
2305 88 0.14764267110936657
2203 1 -0.06687800879061712
2203 2 -0.09215527021260073
2203 3 -0.0942887625584845
2203 4 -0.07248132956993913
2203 5 -0.07454389518671434
2203 6 -0.07376194939747194
2203 7 -0.03748859444086605
2203 8 -0.11050270847167099
2203 9 -0.11327917461283477
2203 10-11 -0.1195660925287001
2203 12-13 -0.13419024016588565
2203 14-15 -0.05062188246008503
2203 16-17 -0.09473553214184705
2203 18-19 -0.13083994771674412
2203 20-21 -0.13543917030205677
2203 22-23 -0.08975775002421216
2203 24-25 -0.10909293285318
2203 26-27 -0.10434495395507071
2203 28-29 -0.13477446201814303
2203 30-31 -0.1335191120622099
2203 32-33 -0.18483312432142185
2203 34-35 -0.16711418038420334
2203 36-37 -0.17470180947108105
2203 38-39 -0.15093523942035603
2203 40-41 -0.1638030991608801
2203 42-43 -0.17221610644612895
2203 44-45 -0.05163970836341747
2203 46-47 -0.03258513578279576
2203 48-49 0.019495616967347473
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2210 24-25 0.46886074432436686
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2215 82-83 7.460162928900615E-4
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2213 10-11 0.09655686329495694
2213 12-13 0.11554195171444803
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2213 26-27 0.1357965302465729
2213 28-29 0.1446503313762264
2213 30-31 0.1711479716283506
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2212 1 0.021057195403479056
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1204 22-23 0.5493165060142999
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1204 26-27 0.5951161670456528
1204 28-29 0.5704500250021454
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1204 32-33 0.6185539282358903
1204 34-35 0.5802755784420768
1204 36-37 0.5073260330900027
1204 38-39 0.5337945281680092
1204 40-41 0.46660867185490673
1204 42-43 0.47599544599028576
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1204 52-53 0.29561303655660254
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1204 56-57 0.19198522289519815
1204 58-59 0.12449862341260598
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1205 12-13 0.4305936661732588
1205 14-15 0.47537011012484953
1205 16-17 0.47958543544719845
1205 18-19 0.4478404449721296
1205 20-21 0.425234477102606
1205 22-23 0.44599990420102387
1205 24-25 0.4395478178567842
1205 26-27 0.4304562365872542
1205 28-29 0.4540280495782838
1205 30-31 0.4661093969668002
1205 32-33 0.48914565698203205
1205 34-35 0.4385459131517493
1205 36-37 0.4134760052393318
1205 38-39 0.37808161490322334
1205 40-41 0.35976236628282265
1205 42-43 0.36380943568507007
1205 44-45 0.3938033061816313
1205 46-47 0.28726403770519227
1205 48-49 0.25526170732669584
1205 50-51 0.3001188439607816
1205 52-53 0.2542968903161267
1205 54-55 0.2067360529913671
1205 56-57 0.194315413524059
1205 58-59 0.1245667011487015
1205 60-61 0.13743233507424435
1205 62-63 0.04739823610405125
1205 64-65 0.010000816034725801
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1205 70-71 -0.3110728168100749
1205 72-73 -0.20941752301060035
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1205 84-85 -0.5621423975778619
1205 86-87 -0.6258627014492539
1205 88 -0.893128459256868
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1210 5 -2.239660235501411
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1210 10-11 0.34710787550915256
1210 12-13 0.37041169079733294
1210 14-15 0.4131087803152269
1210 16-17 0.38458721889060143
1210 18-19 0.4065483046007543
1210 20-21 0.4098662964938171
1210 22-23 0.3979892448299793
1210 24-25 0.45052020377879387
1210 26-27 0.4240752507912475
1210 28-29 0.41733162638448107
1210 30-31 0.436128538399295
1210 32-33 -1.1885893835575274
1210 34-35 -0.021143962942062444
1210 36-37 0.3485524747914326
1210 38-39 0.38308218033801467
1210 40-41 0.3988930085562643
1210 42-43 0.3751675714814908
1210 44-45 0.4144679077499589
1210 46-47 0.3239238504375592
1210 48-49 0.30655812111119474
1210 50-51 0.3841868754754998
1210 52-53 0.29979889167168494
1210 54-55 0.21386106247801706
1210 56-57 0.24607885875756352
1210 58-59 0.21337910108510982
1210 60-61 0.1678414651093263
1210 62-63 0.15262257394807222
1210 64-65 0.06708354024501517
1210 66-67 -0.06925744107775245
1210 68-69 0.0305335367345414
1210 70-71 -0.051642632936591326
1210 72-73 -0.038280952998050566
1210 74-75 -0.06353919344745407
1210 76-77 -0.16323104033938307
1210 78-79 -0.2328597654527229
1210 80-81 -0.34931124133820646
1210 82-83 -1.8232768110171804
1210 84-85 -0.8772029633376874
1210 86-87 -0.6049386812399682
1210 88 -0.5713598534097528
1312 1 0.33169358673464444
1312 2 -0.9037524532215144
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1312 7 0.28872413260258156
1312 8 0.32043993198357157
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1312 10-11 0.39656572639239585
1312 12-13 0.4298510330233185
1312 14-15 -0.6351581082874205
1312 16-17 -0.2782294885868879
1312 18-19 0.28658929856623416
1312 20-21 0.35172658856046723
1312 22-23 0.3147979984226694
1312 24-25 0.3799053373716035
1312 26-27 0.37965347993492315
1312 28-29 0.328380367322481
1312 30-31 0.3569123164630881
1312 32-33 0.4082115704553999
1312 34-35 0.3667886667019147
1312 36-37 0.32889851067110243
1312 38-39 0.30293857568046434
1312 40-41 0.256576893212376
1312 42-43 0.33252050963907465
1312 44-45 0.3581004048830394
1312 46-47 0.3719890750389325
1312 48-49 0.3493223999870736
1312 50-51 0.39276585690442545
1312 52-53 0.28754663044644957
1312 54-55 0.24792177012896133
1312 56-57 0.25441633495487537
1312 58-59 -0.7414777110058708
1312 60-61 -0.7420027520071883
1312 62-63 0.011572535322720512
1312 64-65 0.14658971096944384
1312 66-67 0.028856773018468118
1312 68-69 0.1535982250107537
1312 70-71 0.09112319576630057
1312 72-73 0.0739512057513636
1312 74-75 -0.0035383750421154048
1312 76-77 0.07215861610433905
1312 78-79 0.04823546550058566
1312 80-81 0.05125078256962823
1312 82-83 0.05560309722185863
1312 84-85 0.029374782774141295
1312 86-87 0.05890560429201486
1312 88 0.01191365432747915
1211 1 0.2587694508966649
1211 2 0.26459871852230066
1211 3 0.2389519375410174
1211 4 0.28746056603407766
1211 5 0.37444351389061126
1211 6 0.3482148301146495
1211 7 0.2985320810099026
1211 8 0.32728249641748164
1211 9 0.30323799631676707
1211 10-11 0.3202178376077711
1211 12-13 0.33917549590405827
1211 14-15 0.35696482186980205
1211 16-17 0.33741307410729604
1211 18-19 -0.0591098006026769
1211 20-21 0.20842757112104948
1211 22-23 0.2750106984191163
1211 24-25 -0.42716436344238673
1211 26-27 -0.24501059596307329
1211 28-29 0.19262822778533462
1211 30-31 0.2413965833675178
1211 32-33 0.33214900029155103
1211 34-35 0.2827616791075087
1211 36-37 0.2552415574062863
1211 38-39 0.26907800601451726
1211 40-41 0.2511615830617444
1211 42-43 0.26521435801394233
1211 44-45 0.24558000594534946
1211 46-47 0.2332718887153291
1211 48-49 0.26535898257414914
1211 50-51 0.27067511758875895
1211 52-53 0.23676328456951978
1211 54-55 0.262670257908006
1211 56-57 0.28674602962048823
1211 58-59 -1.3090532682445826
1211 60-61 -1.1466239930261324
1211 62-63 -0.15768743652541417
1211 64-65 0.1238820027716443
1211 66-67 0.30640615710235153
1211 68-69 0.4109645316148409
1211 70-71 0.17094303599661487
1211 72-73 0.0831716966018945
1211 74-75 0.03826950429309761
1211 76-77 0.09443087708486075
1211 78-79 0.11846589522399853
1211 80-81 0.18149011384991098
1211 82-83 0.1643879815601501
1211 84-85 0.18396351545317202
1211 86-87 0.24537998440193576
1211 88 0.23504415916119825
1313 1 0.1735835550286282
1313 2 0.18403418758985168
1313 3 0.14226543608054243
1313 4 0.19009364074982926
1313 5 0.2293424195878444
1313 6 0.26212103431717537
1313 7 0.23940453108815518
1313 8 0.19845474753491743
1313 9 0.22252263559582985
1313 10-11 0.23604673717149183
1313 12-13 0.2242969676471418
1313 14-15 0.25192529035727773
1313 16-17 0.20411886077110353
1313 18-19 -0.8743627434321084
1313 20-21 -0.04208357461375556
1313 22-23 0.1707983997904492
1313 24-25 0.22889983270561487
1313 26-27 0.20401404173380655
1313 28-29 0.1809247321566687
1313 30-31 0.2234231403339635
1313 32-33 0.21337227224216093
1313 34-35 0.17174352840722662
1313 36-37 0.18922040233955073
1313 38-39 0.2298155110362714
1313 40-41 0.23213308580032077
1313 42-43 0.2470910942985043
1313 44-45 0.202942985328896
1313 46-47 0.14742151043839868
1313 48-49 -0.6716453844722423
1313 50-51 -0.5770634339598502
1313 52-53 0.015910235584911447
1313 54-55 0.10272186603383915
1313 56-57 0.20567047833522167
1313 58-59 0.1968249546302161
1313 60-61 0.18996635933933703
1313 62-63 0.17313092068904012
1313 64-65 0.21009083786120186
1313 66-67 0.5945206089715072
1313 68-69 0.5867858188944233
1313 70-71 0.28292183429502415
1313 72-73 0.11277458041758592
1313 74-75 0.07853052519407555
1313 76-77 0.07436970407704635
1313 78-79 0.07633091849931617
1313 80-81 0.0540850761846805
1313 82-83 0.08008412685089539
1313 84-85 0.13194168325182432
1313 86-87 0.10809785125255189
1313 88 0.13420139968637557
1208 1 0.12506794202656124
1208 2 0.12731977710528497
1208 3 0.10221582240532712
1208 4 0.1254415710017014
1208 5 0.19481205571054971
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1208 7 0.15159116522543314
1208 8 0.1606707169747068
1208 9 0.13966695884695213
1208 10-11 0.150107076515539
1208 12-13 0.1647062737384033
1208 14-15 0.1884615534236005
1208 16-17 0.16372748389781577
1208 18-19 0.14891429935529032
1208 20-21 0.1506613738075373
1208 22-23 0.19366495285165541
1208 24-25 0.19113757626777073
1208 26-27 0.11418769120796668
1208 28-29 0.13400117193951644
1208 30-31 0.11505210200544269
1208 32-33 0.1639518488282974
1208 34-35 0.1352819806363783
1208 36-37 0.13230023653477474
1208 38-39 0.169525896917456
1208 40-41 0.19561828799593428
1208 42-43 0.1650183258101734
1208 44-45 0.14098137786770337
1208 46-47 0.1533523878839631
1208 48-49 0.23466391649223795
1208 50-51 0.24312709047656256
1208 52-53 0.2007104990859574
1208 54-55 0.14492033490066802
1208 56-57 0.20380939932864095
1208 58-59 0.2661269824607473
1208 60-61 0.17203596787208397
1208 62-63 0.19197573699562298
1208 64-65 0.16396964897309374
1208 66-67 0.4882848720491495
1208 68-69 0.43775446910354887
1208 70-71 0.14860278997971932
1208 72-73 0.10169336790566774
1208 74-75 0.17705929348257854
1208 76-77 0.1819420489574135
1208 78-79 0.15011198434704198
1208 80-81 0.16200222663004737
1208 82-83 0.20110333395484403
1208 84-85 0.2889677833632298
1208 86-87 0.39307154970194347
1208 88 0.3954238951068554
1314 1 0.0857229915681117
1314 2 0.1004467714179853
1314 3 0.07548633852103848
1314 4 0.14082533617338555
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1314 10-11 0.10211729602040265
1314 12-13 0.12814065877051917
1314 14-15 0.13491902558349267
1314 16-17 0.11803753287509267
1314 18-19 0.13253575902781023
1314 20-21 0.08339606009518263
1314 22-23 0.10842791383975481
1314 24-25 0.020185670931276434
1314 26-27 0.01158671954267021
1314 28-29 0.053085056852623325
1314 30-31 0.08888321657781972
1314 32-33 0.07303856223209237
1314 34-35 0.12118608698378353
1314 36-37 0.17028263101487795
1314 38-39 0.21443434306547005
1314 40-41 0.17462667774420026
1314 42-43 0.16677630535178167
1314 44-45 0.1541243540593129
1314 46-47 0.18644902025553023
1314 48-49 0.23652887006607415
1314 50-51 0.28974772201353716
1314 52-53 0.27610688043525045
1314 54-55 0.2621359155305214
1314 56-57 0.283775635896383
1314 58-59 0.29596146851726246
1314 60-61 0.27991793391662867
1314 62-63 0.2504352201033342
1314 64-65 0.3194710706044148
1314 66-67 0.6898451897004385
1314 68-69 0.7068408910775865
1314 70-71 0.4150739024542318
1314 72-73 0.3279051663495949
1314 74-75 0.26785888319278683
1314 76-77 -0.3116776367346219
1314 78-79 0.12501285507462612
1314 80-81 0.34021137448841543
1314 82-83 0.42943686504084155
1314 84-85 0.5242742254132615
1314 86-87 0.6018247580339988
1314 88 0.6849837270654788
1209 1 0.002957366579053655
1209 2 0.04021325809653575
1209 3 0.004496468138341925
1209 4 0.03572515669771548
1209 5 0.02058961641645851
1209 6 0.042168004751772514
1209 7 0.006531618964586983
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1209 12-13 0.02266074112412042
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1209 16-17 -0.10086278518884484
1209 18-19 0.007894796227979839
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1209 22-23 0.0065801663603153315
1209 24-25 0.025708069404231537
1209 26-27 0.0029339864943054295
1209 28-29 -0.0108199839805323
1209 30-31 -0.023767166195206357
1209 32-33 0.006814809816724221
1209 34-35 0.00446559830230342
1209 36-37 0.03924406057616636
1209 38-39 0.03516526277815757
1209 40-41 0.07009495475636385
1209 42-43 0.013808062626004869
1209 44-45 -0.7723645476976557
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1209 48-49 0.06156483077194963
1209 50-51 0.13815310276913806
1209 52-53 0.11490863140720364
1209 54-55 0.09965995662216898
1209 56-57 0.1449800590082404
1209 58-59 0.22961298698422183
1209 60-61 0.23488961475533898
1209 62-63 0.24452600263943935
1209 64-65 0.26222621292539117
1209 66-67 0.7022059348194603
1209 68-69 0.6079466021262085
1209 70-71 0.2506815351480185
1209 72-73 0.29108858831069995
1209 74-75 0.28316303861778636
1209 76-77 0.3166585346367299
1209 78-79 0.36153241485130394
1209 80-81 0.3568461595605861
1209 82-83 0.34138629149463995
1209 84-85 0.5096281417155168
1209 86-87 0.557137677027125
1209 88 0.6033659343377273
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1315 3 -0.12651596890189154
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1315 50-51 0.0402161717911369
1315 52-53 0.0043924173148610635
1315 54-55 0.07072161237051944
1315 56-57 0.12004512741268059
1315 58-59 0.15227631476724923
1315 60-61 0.11563309938030386
1315 62-63 0.14088050789091966
1315 64-65 0.15898824242079712
1315 66-67 0.607562346509404
1315 68-69 0.5638492191405469
1315 70-71 0.27106605546429563
1315 72-73 0.18966998014187197
1315 74-75 0.20261722530371173
1315 76-77 0.24252428440298957
1315 78-79 0.28844909397186314
1315 80-81 0.36152513289686894
1315 82-83 0.3419352663243451
1315 84-85 0.3912748377917872
1315 86-87 0.5216991237985589
1315 88 0.5102045740096663
1214 1 -0.15327307304831805
1214 2 -0.1492751495185587
1214 3 -0.12726781829820766
1214 4 -0.11390213860689613
1214 5 -0.12621774910871864
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1214 7 -0.15701013083516813
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1214 10-11 -0.15155115127038243
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1214 14-15 -0.14242985392008478
1214 16-17 -0.16026711470964727
1214 18-19 -0.15866906781155166
1214 20-21 -0.18627123854886918
1214 22-23 -0.20348476738700327
1214 24-25 -0.20054071991580003
1214 26-27 -0.19039530713980213
1214 28-29 -0.1845084794044709
1214 30-31 -0.21356222208793696
1214 32-33 -0.20340045905346216
1214 34-35 -0.2087875553516625
1214 36-37 -0.18612647531103477
1214 38-39 -0.16843885654486002
1214 40-41 -0.15185749402122184
1214 42-43 -0.14461036713282027
1214 44-45 -0.1326586542663577
1214 46-47 -0.10138217488252366
1214 48-49 -0.004601259728829632
1214 50-51 -0.03563648512123052
1214 52-53 0.020990507490012078
1214 54-55 0.00991733853384602
1214 56-57 0.058746111112562005
1214 58-59 0.1138130990473698
1214 60-61 0.15342398441407568
1214 62-63 0.1349074673664532
1214 64-65 0.2184848189267754
1214 66-67 0.7573725056099292
1214 68-69 0.6758027627153957
1214 70-71 0.3949613588732461
1214 72-73 0.3223458639860013
1214 74-75 0.3286112987683225
1214 76-77 0.3750043512592214
1214 78-79 0.392920558103377
1214 80-81 0.4424629563996483
1214 82-83 0.46138135058514607
1214 84-85 0.39998599078095864
1214 86-87 -1.8841165625987948
1214 88 -0.4433079009142702
1316 1 -0.22355528558079385
1316 2 -0.1800674994245881
1316 3 -0.21678971339808584
1316 4 -0.1735260656612141
1316 5 -0.20587078457814556
1316 6 -0.20729437613881885
1316 7 -0.2656444809095575
1316 8 -0.2587326798121765
1316 9 -0.2775152495628319
1316 10-11 -0.2509180816053487
1316 12-13 -0.2967092304544181
1316 14-15 -0.27713631381273984
1316 16-17 -0.24120771630654758
1316 18-19 -0.2627733466570774
1316 20-21 -0.2979304799134326
1316 22-23 -0.32595140791385546
1316 24-25 -0.3633289196925986
1316 26-27 -0.3754300637552106
1316 28-29 -0.3836091570233009
1316 30-31 -0.35654825073974195
1316 32-33 -0.3114124246272709
1316 34-35 -0.29886062409870817
1316 36-37 -0.26567889499293074
1316 38-39 -0.27987728105750165
1316 40-41 -0.20610178254339218
1316 42-43 -0.20157587679985767
1316 44-45 -0.20210879640048063
1316 46-47 -0.14224329355931076
1316 48-49 -0.10093957039971713
1316 50-51 -0.07460062097675291
1316 52-53 -0.10135952009288474
1316 54-55 -0.04180642427429149
1316 56-57 0.009063706010472572
1316 58-59 0.06178041344264784
1316 60-61 0.01329528546407488
1316 62-63 0.10425340673519656
1316 64-65 0.15239527769188754
1316 66-67 0.8750167391714996
1316 68-69 0.6698165735975401
1316 70-71 0.3664939165839485
1316 72-73 0.2936231392891031
1316 74-75 0.32396142186764365
1316 76-77 0.3692013424491236
1316 78-79 0.4652068975800603
1316 80-81 0.4399848500779875
1316 82-83 0.5265167025975757
1316 84-85 0.5746551905106649
1316 86-87 0.7665569129785297
1316 88 0.8405224830579101
1215 1 -0.3315561289371729
1215 2 -0.3028048770008738
1215 3 -0.34119845382709
1215 4 -0.5267000666252457
1215 5 -0.49439738574462666
1215 6 -0.44358377912667635
1215 7 -0.4274676143215004
1215 8 -0.4733590562806711
1215 9 -0.8899012634445711
1215 10-11 -0.6221502848622436
1215 12-13 -0.49377014227188454
1215 14-15 -0.42248445567501136
1215 16-17 -0.44274152949027723
1215 18-19 -0.4257442256392707
1215 20-21 -0.43131032252039603
1215 22-23 -0.4610995280943726
1215 24-25 -0.4381764277883846
1215 26-27 -0.44544614168686536
1215 28-29 -0.4176672163282831
1215 30-31 -0.47944365176991965
1215 32-33 -0.4325562002146839
1215 34-35 -0.45063463907474954
1215 36-37 -0.40328606126065125
1215 38-39 -0.40487793235865865
1215 40-41 -0.37216273400810707
1215 42-43 -0.3532800139826051
1215 44-45 -0.34969674284986496
1215 46-47 -0.33127043028824943
1215 48-49 -0.23381956917482682
1215 50-51 -0.21146440526222676
1215 52-53 -0.1880638349697321
1215 54-55 -0.2668890614748065
1215 56-57 -0.15486585217149695
1215 58-59 -0.06796884629905264
1215 60-61 -0.01010164494027066
1215 62-63 -0.024515404337584812
1215 64-65 0.03013693819964658
1215 66-67 0.7907924146165115
1215 68-69 0.652101333456752
1215 70-71 0.3443837700785437
1215 72-73 0.25445958219720666
1215 74-75 0.3260009788798044
1215 76-77 0.44221940414870176
1215 78-79 0.5900602297258857
1215 80-81 0.6329846976068545
1215 82-83 0.6290316425082274
1215 84-85 0.4899211224764173
1215 86-87 0.5875223120151674
1215 88 0.8920602303846117
1212 1 -0.44855643596360295
1212 2 -0.4121977855167529
1212 3 -0.4010284229498353
1212 4 -0.37862411135578355
1212 5 -0.49944695457475063
1212 6 -0.47235174873249264
1212 7 -0.522569517456617
1212 8 -0.49771833822532585
1212 9 -0.4978257579337253
1212 10-11 -0.5211560465591205
1212 12-13 -0.5318224175891615
1212 14-15 -0.5314609640751158
1212 16-17 -0.6059395956084899
1212 18-19 -0.6039167861648949
1212 20-21 -0.6042635005218955
1212 22-23 -0.6024191282333149
1212 24-25 -0.6222574302469539
1212 26-27 -0.5850522294934208
1212 28-29 -0.5936978103116815
1212 30-31 -0.5721352561205819
1212 32-33 -0.520918583111218
1212 34-35 -0.467606130917936
1212 36-37 -0.47037318655237925
1212 38-39 -0.44791874600510795
1212 40-41 -0.38921279415037446
1212 42-43 -0.38537695186163035
1212 44-45 -0.3719407642716064
1212 46-47 -0.40258588356702063
1212 48-49 -0.26994295912918176
1212 50-51 -0.24480785228625734
1212 52-53 -0.21693035853626697
1212 54-55 -0.15553439237556432
1212 56-57 -0.17305812177023228
1212 58-59 -0.03409562987349801
1212 60-61 -0.03800380877250831
1212 62-63 -0.022344653655878233
1212 64-65 0.10841794113257919
1212 66-67 0.8722944315931116
1212 68-69 0.71671401876225
1212 70-71 0.4170605996361161
1212 72-73 0.34915875482852954
1212 74-75 0.525007089781468
1212 76-77 0.5384655873112223
1212 78-79 0.6887986740711831
1212 80-81 0.7814814675544
1212 82-83 0.8517240647680211
1212 84-85 0.8880381316084005
1212 86-87 1.0375819241944626
1212 88 1.1938984950885967
1213 1 -0.5470277513180619
1213 2 -0.5304836038574123
1213 3 -0.5576518572274054
1213 4 -0.5748626420124623
1213 5 -0.7402693934797853
1213 6 -0.743852428814975
1213 7 -0.686769011335592
1213 8 -0.692597095522423
1213 9 -0.7025477266512752
1213 10-11 -0.7265710944555508
1213 12-13 -0.6806069819880207
1213 14-15 -0.711819438278745
1213 16-17 -0.7249045038127662
1213 18-19 -0.7159762942165386
1213 20-21 -0.7070855593668455
1213 22-23 -0.7705966781142521
1213 24-25 -0.7217297515554435
1213 26-27 -0.7560119795619116
1213 28-29 -0.7048650103949683
1213 30-31 -0.6968157957611609
1213 32-33 -0.6306575438404209
1213 34-35 -0.6228468202290074
1213 36-37 -0.5822056305311349
1213 38-39 -0.622568153179067
1213 40-41 -0.6140688960666552
1213 42-43 -0.5407215369042788
1213 44-45 -0.53230312465071
1213 46-47 -0.5074801990186941
1213 48-49 -0.4237256200176631
1213 50-51 -0.3969836202820929
1213 52-53 -0.3575253864201926
1213 54-55 -0.3104008424349658
1213 56-57 -0.26242485157873574
1213 58-59 -0.13999184784100294
1213 60-61 -0.09693778768251349
1213 62-63 -0.05236124915609963
1213 64-65 0.03962889055992491
1213 66-67 0.9381796153983117
1213 68-69 0.7264147221536881
1213 70-71 0.4220605485066429
1213 72-73 0.3212735730663354
1213 74-75 0.4666338711508331
1213 76-77 0.5584758668663028
1213 78-79 0.7294324150614315
1213 80-81 0.8339273571391992
1213 82-83 0.9354934103093093
1213 84-85 0.9983236098441743
1213 86-87 1.197309279379919
1213 88 1.3773559666579622
>>END_MODULE
>>Per sequence quality scores pass
#Quality Count
20 20.0
21 557.0
22 7788.0
23 44472.0
24 130157.0
25 243373.0
26 357296.0
27 470490.0
28 580912.0
29 716865.0
30 896006.0
31 1026449.0
32 1255949.0
33 1689989.0
34 2508659.0
35 3474983.0
36 3526139.0
37 3175113.0
38 3068305.0
39 1335831.0
40 91337.0
>>END_MODULE
>>Per base sequence content fail
#Base G A T C
1 29.795876719075444 25.087046567459186 20.122340221187248 24.994736492278122
2 29.82893162752752 23.044975567758467 16.38148767371972 30.744605130994294
3 32.73111851740744 18.830049075859254 17.79435454859193 30.64447785814138
4 31.563244770776755 18.639542224222165 20.159946733201387 29.637266271799696
5 31.48925603131555 19.772180336759714 18.151172694940296 30.587390936984438
6 32.136615088135095 18.695895223885646 19.059502896211185 30.107986791768077
7 29.078971752740312 19.823019627056777 16.917254921743517 34.1807536984594
8 31.75176915419779 19.132649411051567 16.52777086733677 32.587810567413875
9 30.472917466706456 19.465158589887725 18.07885655559462 31.983067387811197
10-11 31.11201648038564 18.835842582683178 17.18197885155387 32.870162085377316
12-13 32.513631785676374 18.802373219042508 17.481909805505445 31.202085189775673
14-15 30.685181810930683 19.54770679565913 17.89955015089422 31.86756124251596
16-17 31.346452208972348 19.0303628180849 16.493217325447283 33.12996764749547
18-19 30.61170647543608 19.82758881829579 16.045843652768163 33.51486105349996
20-21 31.70404322904122 19.424640695011668 15.701108578452475 33.170207497494644
22-23 31.69168454122562 19.161401105102897 15.869730604832446 33.27718374883904
24-25 30.891752073157953 19.17327517294489 17.777852231797905 32.157120522099255
26-27 34.182572711034744 18.443590592879207 16.483362733994078 30.890473962091967
28-29 33.84950976031245 18.81191802488295 16.22950942123286 31.109062793571745
30-31 33.653123489980565 19.04279566199506 15.27132380282784 32.032757045196526
32-33 32.84930871969495 20.790182599205476 15.989627470869621 30.37088121022995
34-35 29.820942780034365 21.643511084332083 15.244622211672496 33.29092392396106
36-37 30.433544692139414 19.63206213874723 15.070311897258257 34.8640812718551
38-39 33.66022811987977 19.192976588881017 15.371496795430875 31.775298495808336
40-41 34.54609691884321 19.730271358951786 15.663917487638749 30.059714234566254
42-43 33.697874524215486 19.965272019393133 14.669186641181339 31.667666815210044
44-45 34.226040967506925 20.132343714093956 14.402479662633255 31.239135655765864
46-47 33.897974598104724 19.90508980074464 14.930603184310517 31.266332416840115
48-49 33.994550999102984 19.308121210724867 14.975025974249059 31.72230181592309
50-51 35.115355002725664 19.168278306646375 14.719538880399401 30.99682781022856
52-53 34.132130814915456 19.44315672576014 15.61846661112664 30.80624584819776
54-55 32.74135771888787 19.542704152365275 15.310004136428864 32.40593399231799
56-57 33.3062731902764 19.5223113797454 15.11905172904178 32.05236370093642
58-59 34.62683236366178 18.271847938996245 14.733659833711455 32.36765986363052
60-61 35.04508173463357 17.934298130713024 15.349478427400248 31.67114170725316
62-63 34.15420931180733 18.77269687912639 15.394711544797396 31.67838226426889
64-65 33.413916029074784 19.433784093815056 15.862229810069897 31.29007006704026
66-67 32.035782385682474 19.752032590740626 14.351316143161307 33.860868880415595
68-69 33.03637704570495 18.6557233611889 14.835836518020695 33.47206307508545
70-71 30.65306463682276 19.10030282101684 14.564894012644105 35.6817385295163
72-73 33.94753878361882 18.083699257194827 14.241381854466376 33.72738010471998
74-75 33.77360059809861 18.584289360165897 14.797360364979026 32.844749676756464
76-77 32.828136846285794 19.720314118339253 14.26310440966948 33.18844462570548
78-79 31.034052678982892 19.28004895849935 14.751603979772495 34.93429438274526
80-81 29.946667196572964 19.17997687288499 15.45309304828493 35.42026288225712
82-83 29.780032955963538 18.651801566659206 15.165679771675872 36.40248570570139
84-85 30.313573955250256 19.21509060824573 13.68106313455347 36.790272301950544
86-87 30.475293232354417 19.62475714289009 13.18887076097589 36.711078863779605
88 31.438514178217087 19.95288801912023 13.77040114455171 34.838196658110974
>>END_MODULE
>>Per sequence GC content fail
#GC Content Count
0 1187.0
1 737.0
2 252.0
3 200.5
4 164.5
5 134.0
6 113.0
7 99.0
8 92.5
9 103.0
10 122.0
11 137.5
12 202.0
13 278.0
14 388.5
15 608.5
16 927.5
17 1117.0
18 1475.5
19 2389.0
20 3899.0
21 6023.0
22 11264.5
23 19700.0
24 28571.0
25 33148.5
26 36584.5
27 48363.5
28 60330.0
29 75063.5
30 84317.0
31 97138.0
32 124801.5
33 137908.5
34 143306.5
35 157602.5
36 175132.5
37 195324.0
38 206398.5
39 217409.5
40 245161.0
41 320649.0
42 380999.5
43 383128.5
44 378899.0
45 379181.5
46 433786.5
47 493772.5
48 517858.0
49 538636.0
50 546147.0
51 531089.5
52 519735.0
53 527019.5
54 518625.0
55 510702.0
56 492891.5
57 478640.0
58 486181.0
59 503871.0
60 491543.0
61 485678.5
62 540534.5
63 487107.0
64 379303.0
65 377658.5
66 407321.0
67 415171.5
68 475542.5
69 551220.0
70 493262.5
71 427563.0
72 393683.0
73 413048.5
74 381694.5
75 288744.5
76 424983.5
77 663641.5
78 844658.0
79 882572.5
80 916317.5
81 1139153.5
82 1079135.5
83 870216.5
84 863857.0
85 743772.0
86 585408.0
87 458820.0
88 248185.0
89 128712.0
90 83459.0
91 33730.5
92 37639.0
93 25831.0
94 9969.5
95 7088.5
96 5522.0
97 1749.0
98 577.0
99 175.0
100 26.0
>>END_MODULE
>>Per base N content pass
#Base N-Count
1 0.008434722765906158
2 0.0
3 0.0
4 0.0
5 1.6259706536686573E-5
6 0.0011137898977630302
7 0.0
8 0.0
9 0.0
10-11 3.658512567082131E-5
12-13 1.8292989297219124E-5
14-15 0.0
16-17 3.0492646515336945E-5
18-19 0.0
20-21 1.6266560120697877E-5
22-23 1.2202450862255685E-5
24-25 0.0011269689153900625
26-27 0.0011801634705465992
28-29 3.704217122971603E-4
30-31 0.0011767006831561791
32-33 0.0038997083791964837
34-35 0.0032613723708266353
36-37 9.108863574308693E-4
38-39 8.153871879311934E-4
40-41 0.0
42-43 2.366244268946181E-4
44-45 9.284915025069882E-4
46-47 9.493053004226674E-4
48-49 7.924924817892683E-4
50-51 0.0018698659152871042
52-53 0.0
54-55 1.9437106311641714E-4
56-57 0.00883497549558633
58-59 2.233678923081504E-4
60-61 2.6662165546206253E-5
62-63 0.0
64-65 0.0021842496572289684
66-67 0.0033655766271647536
68-69 0.004429210832824245
70-71 5.559718480566004E-4
72-73 2.0591549928022235E-6
74-75 7.845380522576472E-4
76-77 2.6563099407148685E-4
78-79 6.17746497840667E-5
80-81 0.0032225775637354805
82-83 3.500563487763781E-5
84-85 8.751408719409451E-4
86-87 1.5237746946736454E-4
88 0.0020921014726870595
>>END_MODULE
>>Sequence Length Distribution warn
#Length Count
6-7 234.0
8-9 219.0
10-11 361.0
12-13 1362.0
14-15 1844.0
16-17 2242.0
18-19 3042.0
20-21 4659.0
22-23 5935.0
24-25 6362.0
26-27 6382.0
28-29 6169.0
30-31 6867.0
32-33 8029.0
34-35 8272.0
36-37 8435.0
38-39 8380.0
40-41 8314.0
42-43 8965.0
44-45 10153.0
46-47 11715.0
48-49 12344.0
50-51 13556.0
52-53 14856.0
54-55 17413.0
56-57 20538.0
58-59 19839.0
60-61 20988.0
62-63 23075.0
64-65 27898.0
66-67 30139.0
68-69 298.0
70-71 0.0
72-73 0.0
74-75 0.0
76-77 0.0
78-79 0.0
80-81 0.0
82-83 0.0
84-85 0.0
86-87 0.0
88-89 2.4281805E7
>>END_MODULE
>>Sequence Duplication Levels fail
#Total Deduplicated Percentage 24.78789062862651
#Duplication Level Percentage of deduplicated Percentage of total
1 84.61511715073699 20.97430269460888
2 8.757229770533735 4.3414650752348445
3 2.789148680994106 2.0741133735437933
4 1.1479382354554852 1.1381986971555629
5 0.6627919956322319 0.8214607748630433
6 0.34893507315456146 0.5189618657908236
7 0.25346391174482735 0.4397985005844236
8 0.207295633919247 0.4110737201105676
9 0.16814620022249155 0.3751190658660829
>10 0.7841934438814446 3.7305440819847697
>50 0.09431069534716853 1.6750078587804844
>100 0.10905020865894283 6.029419404598243
>500 0.025234092310565028 4.437640933431968
>1k 0.02921964535696695 15.293666806624499
>5k 0.003990854974120789 6.839268086531241
>10k+ 0.0039344070772215104 30.89995906029056
>>END_MODULE
>>Overrepresented sequences warn
#Sequence Count Percentage Possible Source
TAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGG 231674 0.9417378130450812 No Hit
TAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGG 203234 0.8261312995692396 No Hit
GGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGG 184047 0.7481375522393885 No Hit
GTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGA 177028 0.7196058321941377 No Hit
GTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGC 143987 0.5852965912744723 No Hit
GCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGG 141495 0.5751667941021167 No Hit
GACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGAC 138738 0.5639597913717054 No Hit
AAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGC 125388 0.509693020805514 No Hit
GCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCG 120773 0.49093338438881184 No Hit
TCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGG 114058 0.46363740204034926 No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG 113698 0.46217402845204747 No Hit
CGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGC 109668 0.4457923741163358 No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG 106388 0.43245941475625277 No Hit
AACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACC 99735 0.4054154578591088 No Hit
ACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCG 92228 0.3749000536163823 No Hit
AGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAA 91811 0.37320497920993273 No Hit
TCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGG 89594 0.3641930368619742 No Hit
CGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGG 87532 0.3558111581423123 No Hit
ACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCGCGCCGCCGCG 84577 0.3437992999383351 No Hit
CCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGA 81783 0.33244189492245946 No Hit
AGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGT 78416 0.31875528694520355 No Hit
CGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATGAGAGTAG 76276 0.3100563439480763 No Hit
TAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCG 74348 0.3022191653973933 No Hit
CCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTC 72494 0.2946827914176391 No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA 72285 0.2938332217510972 No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA 71477 0.29054876103068655 No Hit
GGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCG 59624 0.24236718563585008 No Hit
TGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGA 58785 0.23895671218978004 No Hit
TCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAAC 58644 0.23838355753436186 No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG 56908 0.23132684489743988 No Hit
GCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGG 56242 0.22861960375908152 No Hit
GTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAG 56203 0.22846107162034887 No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA 55723 0.2265099068359465 No Hit
CCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCG 54612 0.22199377334538176 No Hit
TTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTG 54079 0.2198271674493683 No Hit
ATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAG 52756 0.2144492695123592 No Hit
GTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGC 52548 0.2136037647724515 No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC 52292 0.21256314355410358 No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG 50700 0.2060917803525023 No Hit
CACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCG 49447 0.20099842727988523 No Hit
CGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCC 47321 0.1923563932556363 No Hit
TCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTAT 45137 0.18347859348660545 No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA 45094 0.18330380164133608 No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC 44626 0.18140141597654375 No Hit
GGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGC 44589 0.1812510136910794 No Hit
GTGTTTCAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTG 43201 0.17560889552284917 No Hit
CAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGG 42643 0.1733406664609814 No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC 40755 0.16566608497566532 No Hit
GCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGAC 40749 0.16564169541586027 No Hit
AGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCG 39145 0.15912155309464895 No Hit
GCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCTCG 37859 0.15389405744310425 No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG 37726 0.15335342220075943 No Hit
GCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGC 37464 0.15228841142260643 No Hit
GCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCG 37320 0.15170306198728573 No Hit
GCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGT 36333 0.14769097939935832 No Hit
AGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCA 36274 0.14745114872794218 No Hit
CGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACG 36107 0.14677230598003552 No Hit
CCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT 35785 0.14546339960383226 No Hit
CCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAAC 35108 0.14271144427249804 No Hit
GCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCG 33917 0.1378701166511996 No Hit
TCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGAC 33612 0.13663031402777726 No Hit
CGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCT 33390 0.13572790031499116 No Hit
ACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCC 33217 0.13502466800727947 No Hit
CCGCCAGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTA 32867 0.13360194368531939 No Hit
ACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTA 32771 0.1332117107284389 No Hit
CCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGG 32563 0.13236620598853122 No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCCCGCCCCC 32417 0.13177272669994217 No Hit
GGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACG 32286 0.13124022131086566 No Hit
CCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCGCGCCGCCGCGG 32261 0.1311385981450114 No Hit
ACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGG 32079 0.13039878149759213 No Hit
CACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG 31640 0.1286142787051908 No Hit
CGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGG 31518 0.12811835765582186 No Hit
CGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACGCGCGGG 30507 0.12400871682867433 No Hit
CTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGG 30454 0.12379327571706321 No Hit
CGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCCCGGGG 30079 0.12226892822924885 No Hit
TATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGA 29874 0.12143561826924366 No Hit
CCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCC 29508 0.11994785512113686 No Hit
CAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACT 29421 0.11959420650396392 No Hit
GAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCCC 29353 0.11931779149284025 No Hit
GGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATGAGAGTAGTG 29253 0.11891129882942307 No Hit
GGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCC 29209 0.11873244205751954 No Hit
CTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACC 28568 0.1161268240850155 No Hit
ACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCCCGGG 28096 0.11420817871368649 No Hit
GCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGAC 27674 0.11249277967406605 No Hit
ATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGAC 27656 0.11241961099465096 No Hit
CCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTC 27242 0.1107367313681039 No Hit
GTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAG 27138 0.11031397899815006 No Hit
ACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCG 26942 0.10951725337785241 No Hit
CGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGAC 26793 0.10891157930936085 No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG 24623 0.10009068851320838 No Hit
>>END_MODULE
>>Adapter Content pass
#Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence
1 4.064926634171643E-6 0.0 4.064926634171643E-6
2 4.064926634171643E-6 0.0 8.129853268343287E-6
3 4.064926634171643E-6 0.0 8.129853268343287E-6
4 8.129853268343287E-6 0.0 8.129853268343287E-6
5 1.219477990251493E-5 0.0 1.6259706536686573E-5
6 2.0324633170858216E-5 0.0 2.0324633170858216E-5
7 2.0324633170858216E-5 0.0 2.0324633170858216E-5
8 2.438955980502986E-5 0.0 2.8454486439201503E-5
9 2.438955980502986E-5 0.0 2.8454486439201503E-5
10-11 2.8454486439201503E-5 0.0 2.8454486439201503E-5
12-13 4.268172965880226E-5 0.0 2.8454486439201503E-5
14-15 4.4714192975888076E-5 0.0 3.2519413073373146E-5
16-17 7.316867941508958E-5 0.0 3.455187639045897E-5
18-19 1.0162316585429107E-4 0.0 3.658433970754479E-5
20-21 1.2601272565932093E-4 0.0 3.658433970754479E-5
22-23 1.605646020497799E-4 0.0 4.064926634171643E-5
24-25 2.2357096487944037E-4 0.0 5.487650956131718E-5
26-27 2.7031762117241424E-4 0.0 5.894143619548883E-5
28-29 3.069019608799591E-4 0.0 6.300636282966047E-5
30-31 3.617784704412762E-4 0.0 6.910375278091793E-5
32-33 4.369796131734517E-4 0.0 6.910375278091793E-5
34-35 5.081158292714554E-4 0.0 7.113621609800375E-5
36-37 5.609598755156868E-4 0.0 7.723360604926122E-5
38-39 6.25998701662433E-4 0.0 7.723360604926122E-5
40-41 7.032323077116943E-4 0.0 8.129853268343287E-5
42-43 8.007905469318136E-4 0.0 8.53634593176045E-5
44-45 9.268032725911347E-4 0.0 9.349331258594779E-5
46-47 0.001014199195225825 0.0 9.349331258594779E-5
48-49 0.00113817945756806 0.0 9.755823922011944E-5
50-51 0.0012377701601052654 0.0 1.1178548243972019E-4
52-53 0.0013861399822525304 0.0 1.1585040907389184E-4
54-55 0.001550769510936482 0.0 1.1788287239097765E-4
56-57 0.0016910094798154034 0.0 1.1991533570806347E-4
58-59 0.0018454766919139259 0.0 1.219477990251493E-4
60-61 0.001947099857768217 0.0 1.219477990251493E-4
62-63 0.0020344957804029075 0.0 1.219477990251493E-4
64-65 0.002174735749281829 0.0 1.2804518897640676E-4
66-67 0.0025791959493819076 0.0 1.3007765229349258E-4
68-69 0.06475834620898845 0.0 1.3007765229349258E-4
70-71 0.17006230313052195 0.0 1.3617504224475006E-4
72-73 0.3245823592752886 0.0 1.422724321960075E-4
74-75 0.5018334851583431 0.0 1.4430489551309333E-4
76 0.6620220814944622 0.0 1.4633735883017916E-4
>>END_MODULE
>>Kmer Content fail
#Sequence Count PValue Obs/Exp Max Max Obs/Exp Position
CGTAGCG 15975 0.0 76.11031 2
GCGTAGC 16410 0.0 74.197815 1
GTAGCGT 19035 0.0 63.896618 3
TCGACTG 2300 0.0 57.04888 1
CGGGCTC 15660 0.0 44.157978 1
GGGCTCG 15755 0.0 44.0435 2
TAGCGTC 39575 0.0 42.24737 1
AGCGTCC 41160 0.0 40.557964 2
TTGCCCA 12440 0.0 37.89938 1
GTCCGCA 32375 0.0 37.533318 1
GGTCCGC 18010 0.0 37.17182 5
GCGTCCG 45370 0.0 36.740677 3
GGCCGCG 44730 0.0 36.656185 4
TCGTGCG 445 0.0 36.628494 1
CGTCCGC 49440 0.0 33.716248 4
CCCTGGT 20065 0.0 33.529495 1
TCCGCGG 50005 0.0 33.490456 6
CCGCGAC 49660 0.0 32.377476 6
GCCGCGA 49840 0.0 32.366627 5
CGCCTAG 6660 0.0 29.692919 82
>>END_MODULE
